Fig. 10. Proliferating cells at E12.5 and E16.5 in Gsh2 and Gsh1/2 double homozygous mutants. Immunohistochemistry for the cell cycle marker Ki67 shows strong labeling in the VZ and SVZ (separated by the dashed line) of the wild-type LGE and MGE (A). Labeling in the Gsh2 mutant is normal in the VZ and MGE SVZ but significantly lower in the LGE SVZ (B). The Gsh1/2 double mutant (C) is similar to the Gsh2 mutant. At E16.5, the SVZ of the Gsh2 mutant LGE (E) has improved, showing numerous Ki67-positive cells which is not the case in the SVZ of the double mutant LGE (E).