Fig. 4. Localization of ankyrin G and paranodin in jimpy mice. Sections from corpus callosum of wild-type (A,C) or jimpy (B,D) littermates were examined by double immunostaining with antibodies against paranodin (green) and ankyrin G (red) at postnatal day 15 (A,B) or 21 (C,D). In wild-type mice, paranodin immunoreactivity was concentrated in paranodes, in most instances flanking the nodal aggregates of ankyrin G on either side (A,C). In jimpy mice, paranodin immunoreactivity was diffuse, along the axonal membranes (arrows), with no identifiable paranodal cluster. Although ankyrin G clusters were similar in both genotypes at P15, their limits appeared less well delineated in jimpy mice at P21. Scale bar: 10 µm.