Fig. 4. Bone abnormalities in LAP/Acp5-deficient mice. (A-D) Radiographs of 8-week-old mice: (A) control; (B) Lap^–/–; (C) Acp5^–/–; and (D) Lap^–/–/Acp5^–/–. Note the foreshortening of long bones in the LAP/Acp5-deficient mouse. (E-H) Light microscopy of growth plates of 3-month-old mice: (E) control; (F) Lap^–/–; (G) Acp5^–/– and (H) Lap^–/–/Acp5^–/–. Note the expansion of the cartilaginous growth plates (egp) in Acp5- and LAP/Acp5-deficient mice with disruption, hyperplasia and hypertrophy of the chondrocytes (ps, primary spongiosa). (I,J) PMMA embedded distal femurs of control and Lap^–/–/ Acp5^–/– after plasma ashing. Imaged with three detectors, used to give red, green and blue signal components. Colour here codes for direction and slope of the internal surfaces exposed by removing PMMA. Control image field is 4 mm in height. (K,L) Localisation of succinylated wheat germ agglutinin (sWGA)-binding sites in growth plates of (K) control bone and (L) LAP/Acp5 doubly deficient bone. Scale bars: 120 µm in E-H; 50 µm in K,L.