Fig. 3. (A) The Muv phenotype in lin-40(s1675) animals. P(5-7).p have formed a normal vulval invagination, and P8.p was ectopically induced and its progeny formed a pseudovulva in this animal. Arrows point to vulval invaginations formed by induced Pn.ps. (B) The ‘multiprotrusion’ phenotype in a lin-37(n758); lin-40(ku285) double mutant. Note that each of the P(5-7).p cells formed a vulval invagination in this animal. (C,E) jam-1::gfp expression in wild-type animals during the L2 stage. Arrowheads point to the boundaries between neighboring Pn.ps and arrows indicate the nuclei of Pn.ps. JAM-1::GFP can be detected on the apical surface of Pn.ps where they adjoin the hypodermal syncytium. Note that in E, but not in C, P3.p is already fused to hyp7. (D,F) DIC Nomarski images of the worms shown in C,E. Numbers next to arrows represent the identity of Pn.ps. (G) lin-39::lacZ expression in wild-type animals. Arrows point to the MH27 staining on the surface of Pn.ps and arrowheads indicate the nuclei with positive anti-ß-galactosidase staining. Ventral is downwards and anterior is towards the left. Scale bars: 10 µm.