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Fig. 3. A single injection of endo N removes PSA for at least 24 hours. (A) PSA staining in 32 hpf zebrafish embryo. (B,C) Zebrafish embryo at 32 hpf that received an injection of Endo N into the ventricle at 20 hpf, double labelled with PSA and acetylated {alpha}-tubulin antibodies. (B) The specific PSA labeling has disappeared and only a faint overall fluorescence remains. (C) The tubulin label demonstrates that most longitudinal and commissural axon tracts appear normal, indicating that PSA removal does not interfere with overall development of the zebrafish nervous system. ac, anterior commissure; llf, lateral longitudinal fascicle; mlf, medial longitudinal fascicle; pc, posterior commissure; poc, postoptic commissure. (A-C) Confocal images, dorsal view, rostral at the top. Scale bar: 50 µm.