Fig. 8. Speed of inhibition of DIF-1 synthesis by DIF-1 in living cells. Cells of strain V12M2 were incubated in submerged culture with cAMP and 36Cl–. At 12 hours, fresh medium (still containing 36Cl–) was substituted, with 100 nM DIF-1 added as indicated. Labelled compounds were extracted from cells and media, resolved by TLC and quantitated using a Phosphorimager. Data are typical of three experiments.