Fig. 8. Blocking PDGFR tyrosine kinase do not interfere with development of Plp⁺ progenitors cells. Rostral (A) or caudal (B,C) telencephalon from E12.5 OF1 wild-type (A,B), or plp-sh ble-lacZ transgenic (C) mouse were dissociated, seeded in 96 wells dishes and cultivated in BS medium supplemented with 1% FCS. After 2 days in vitro, either STI571 (50-500 nM) or PDGF-AA (10 ng/ml) was added. After 13 (A,B) or 12 (C) days in vitro, cultures were immunolabeled with O4 mAb alone (A,B), or O4 mAb and anti-ß-galactosidase Ab (C), and immunopositive cells were counted. In C, the white columns represent the O4⁺ cells and the hatched columns the ß-gal⁺ cells. Each column represents the mean±s.e.m. of three separate experiments representing 14 to 24 different cultures (A,B) or two separate experiments representing eight to 19 different cultures (C) (***P<0.0001; * P<0.05, Student’s t-test).