Fig. 2. Amphiphysin is postsynaptic at the larval neuromuscular junction. (A-D) Amphiphysin (green) does not colocalize with presynaptic marker Cysteine String Protein (red) at third instar neuromuscular synapses; Amph staining surrounds the CSP staining, as expected for a postsynaptic protein. Low-magnification view of the NMJs at muscle 6 and 7 of abdominal segment 2 showing Amph (A), Csp (B) and the merged image (C); a high-magnification image of the region indicated with an arrow in C is shown in D. (E-H) Amphiphysin (green) is precisely colocalized with the postsynaptic marker Discs large (red) at third instar type I neuromuscular synapses. Low-magnification view showing Amph (E), Dlg (F) and the merged image (G); a high-magnification image of the region indicated with an arrow in G is shown in H.