Fig. 1. unc-83 mutations disrupt the nuclear migrations of three cell types. (A) Left lateral view of P-cell nuclear migration in wild-type and unc-83 embryos at 25°C. P-cell cytoplasm is gray and nuclei are white. White X marks dying nuclei. Ventral is downwards. (B-C) Expression of UNC-47::GFP in GABAergic neurons in the ventral cords of adult hermaphrodites. Ventral is downwards, anterior is leftwards. (B) Wild type, showing the 19 neural cell bodies scored between the arrows. (C) unc-83(e1408), with only 11 GABAergic neurons between the arrows. (D) The dorsal surface of a pre-elongation embryo illustrating intercalation and nuclear migration of hyp7 precursors in wild-type and unc-83 embryos. Cytoplasm of the hyp7 precursors is gray, nuclei that migrate from right to left are black, and nuclei that migrate from left to right are white. Anterior towards left, right is upwards. (E,F) Lateral view of L1 hermaphrodites. Dorsal is upwards. (E) Wild type showing no nuclei in the dorsal cord. (F) An unc-83(e1408) hermaphrodite. The arrows mark misplaced hyp7 nuclei in the dorsal cord. (G) A dorsal view, through the middle of a pre-elongation embryo, of nuclear migration during intestinal polarization during the E16 stage in wild-type and unc-83 embryos raised at 15°C. Cytoplasm of embryonic intestinal cells is gray, nuclei right of the midline are black and nuclei left of the midline are white. Anterior is leftwards, right is upwards. (H) Wild-type embryonic intestinal cells. (I) unc-83(e1408) embryonic intestinal cells. M marks the midline where the intestinal lumen will form, the arrows mark examples of intestinal nuclei at the mid-line in wild-type and separated away from the midline in the unc-83(e1408) embryo. Scale bars: 100 µm for B,C; 10 µm for E,F; 10 µm for H,I.