Fig. 4. Cloning unc-83. (A) The genomic region on chromosome V between exp-2 and dpy-11 is shown. The names of cosmids in the region that rescue exp-2 or dpy-11 or that contain part of unc-83 are indicated. (B) Cosmid W01A11 and its derivatives are shown on the left. The deleted parts of W01A11 in pD19 and pD21 are indicated by broken lines. The numbers above the lines are the sites of breakpoints of the deletions of W01A11 in pD19 and pD21 and of the region of W01A11 subcloned into pBS to create pD22 (see Materials and Methods). On the right are data concerning the rescue of the unc-83(e1408) nuclear migration defect of hyp7 cells and P cells. (C) The genomic regions of three predicted transcripts of unc-83 are shown. The white boxes represent exons, which are connected by introns. The predicted translational start site (ATG) in each transcript and any detected SL1 splice leader sequences of each transcript are indicated. The numbers at the bottom label exons 1 to 16 of the longest unc-83 transcript. (D) The molecular lesions of 16 unc-83 alleles are shown. The arrows indicate the approximate sites of the lesions. For nonsense mutations, the affected amino acid is numbered. Black boxes indicate alleles that did not disrupt hyp7 nuclear migration, while a white background indicates alleles that disrupted both P cell and hyp7 nuclear migrations. Sequence data for the unc-83 transcripts are available from GenBank/EMBL/DDBJ under Accession Number AF338767.