Fig. 7. UNC-84 is required to target UNC-83 to the nuclear envelope. (A-D) Localization of UNC-83 antibody in pre-elongation embryos in the four classes of unc-84 embryos. Anterior is leftwards. (A) unc-84(n369), a class 1 or null allele. (B) unc-84(n371), a class 2 allele. (C) unc-84(n399), a class 3 allele. (D) unc-84(e1411), a class 4 allele. Scale bar: 10 µm. (E) The molecular lesions in UNC-84 of the seven alleles tested for UNC-83 localization. Only one allele from each class is shown in A-D, although other alleles were tested (see text). (F) UNC-83 and the SUN domain of UNC-84 interact in vitro. The ³⁵S-methionine-labeled UNC-83 that was pulled down by the bait-coated beads is shown. Labels designate the GST fusion protein attached to beads used as bait, or the presence of cold UNC-83/MBP in the middle lane.