Fig. 3. Neural patterning in OVE1070 transgenic eyes. In E15.5 OVE1070 embryos, BrdU incorporation was detected in a high percentage of the cells in the RPE-derived neural retina (rNR), analogous to the original neural retina (NR) (A). Very few cells in the unaffected ventral RPE were actively proliferating (A). Immunohistochemistry with antibody to neuron-specific ß-tubulin III showed that retinal ganglion cells (g) had been generated in both the NR and rNR at E15.5 (B). The tissue morphology and the absence of ganglion cells in the anterior margin between NR and rNR indicate that this tissue was not converted to neural retina. At postnatal day 6 (C), both the NR and rNR became laminated and formed three distinct cellular layers referred to as the ganglion cell layer (g), the inner nuclear layer (i) and the outer nuclear layer (o). Immunohistochemistry with antibody to rhodopsin showed that photoreceptors were born and located basally in both retinae (C). Abbreviations: L, lens; Scale bars: 50 µm in C; 100 µm in A,B.