Fig. 10. Representative frozen sections of neonatal lenses counterstained with Hoechst dye (A) or immunolabelled for phospho-ERK (B) or phospho-p38 (C). Strong punctate nuclear reactivity for phospho-ERK was detected throughout the epithelial cells, extending into the transitional zone where cells are elongating into fibres (A,B). Fibre cells deeper in the cortex demonstrated a marked reduction in labelling with no reactivity apparent in these maturing cells (A,B, arrows). No labelling for phospho-p38 was detected in the lens (C). Abbreviations: le, lens epithelium; lf, lens fibres. Scale bar: 40 µm.