Fig. 8. Replication initiation proteins are mis-localized in E2f2 mutant follicle cell nuclei. Ovaries were dissected, fixed and treated with anti-ORC2 (left panels), anti-ORC5 (middle panels) or anti-CDC45L (right panels) antibodies. An image of a single nucleus from a stage 10B egg chamber is shown in each panel. (A) yw⁶⁷ wild type. These three proteins are detected in a focus coincident with the third chromosome chorion cluster. (B) Df(2L)E2f2³²⁹/Df(2L)E2f2³²⁹; P[E2f2^–; Mpp6⁺]/+. In the mutant, these replication proteins are not localized. (C) Df(2L)E2f2³²⁹/Df(2L)E2f2³²⁹; P[E2f2⁺; Mpp6⁺]/+. Mis-localization in the mutant is rescued by a wild-type E2f2 transgene.