Fig. 9. Gene expression analysis in E2f2 mutant follicle cells. Total RNA was isolated from intact follicle cells prepared by trypsin dissociation of dissected ovaries and subjected to RT-PCR analysis with gene specific primers. Left panels are from yw⁶⁷ wild-type RNA, and right panels are from Df(2L)E2f2³²⁹/Df(2L)DS8, P[E2f2^1-188; Mpp6⁺] RNA. RT-PCR reactions were sampled at cycle 22 (lane 1), cycle 25 (lane 2), cycle 30 (lane 3) and cycle 40 (lane 4) for ORC2, ORC5, ORC1, RNR2 and PCNA, and cycles 5, 10, 15 and 20, for rp49 (lanes 1-4, respectively).