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Fig. 3. Removal of Smo activity abolishes Hh signaling in the cartilage. In situ hybridization with 35S-labeled probes for Ptc1 and Gli1 of tibial sections of Smon/Smoc embryos that carry either Cre15 or Cre10 transgenes, compared with wild-type and Ihh mutant embryos at E14.5 and E18.5.