Fig. 6. Loss of Gli2 does not rescue the Shh mutant defects and expression of Gli1 from the Gli2 allele in the neural plate does not rescue the ventral spinal cord defects in Shh mutant embryos. Antibody staining of E10.5 embryos for Isl1/2, HNF3ß and Nkx2.2. In wild-type embryos, Nkx2.2-expressing interneurons and Isl1/2-expressing motoneurons are expressed in the ventralateral spinal cord (A,E), and HNF3ß is expressed in the floor plate (I). These markers are not expressed in Shh^–/– (B,F,J) or Gli2^–/–;Shh^–/– embryos (C,G,K). In addition, expression of one copy of Gli1 in the place of Gli2 in Shh mutant embryos does not rescue these ventral cell types (D,H,L). Scale bar: 50 µm.