Fig. 2. Abnormal appearance of the kidneys of plc-1^–/– chimeric mouse. (A) Abdominal cavities of the control (right) and plc-1^–/– (left) chimeras. (B) Histopathology of the kidney from plc-1^–/– chimeric mice. The specimens of the kidney from plc-1^–/– chimeric mice were stained with Haematoxylin and Eosin. (C) In situ hybridization of the kidney with a Y-chromosome-specific probe. Dark spots show digoxigenin-positive plc-1^–/– cells. *Normal looking tubules were negative for digoxigenin signals. (D,E) Representative kidney glomeruli in the plc-1^–/– and control chimeras. The arrow indicates a sclerotic glomeruli.