Fig. 6. In vitro differentiation of the wild-type (+/+), plc-1^+/– (+/–) and plc-1^–/– (–/–) ES cells. (A) ES cells differentiated into primitive erythrocytes (Ery^P) and definitive erythrocytes (Ery^D) with or without erythropoietin (EPO). Ery^P production at the 7th day (left) and Ery^D production at the 13th day (right) were investigated. (B) ES cells were differentiated to monocytes/macrophage with or without macrophage colony-stimulating factor (M-CSF) by co-culture with the stromal cell line, OP9. Total numbers of adherent cell on the tenthth day were counted. Data represent mean±s.e.m. from six independent experiments.