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Fig. 8. Histological analyses of adherent bone marrow cultures. (A) Three cell morphologies are discernible: larger polygonal fibroblast-like cells (black arrow), smaller stellate myeloid cells (red arrow), and small round endothelial-like cells (purple arrow). (B-D) Immunostaining of cultures with anti-mouse T1{alpha} antibody (brown staining; B, arrows). (C) At higher magnification, the T1{alpha}-positive cell demonstrates fibroblast-like morphology. (D) Staining within a cell colony is heterogeneous. Bars, 20 µm (A); 200 µm (B); 15 µm (C); 25 µm (D).