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Fig. 1. (A-E) Expression of the control R{alpha}2AchnlsLacZ transgene (A,C) and of the R{alpha}2AChnlsLaacZ transgene (B,D,E) in E11.5 embryos. (A,E) in toto X-Gal staining, (B) whole-mount in situ hybridization of a {alpha}-2 embryo with a LaacZ probe, (C-D) transverse sections of X-Gal stained embryos. (F-K) Examples of monosegmented unilateral clones. (F-H) In toto; (I-K) transverse sections. Clones VG24 (F,I) and VG28 (G,J) are restricted to the medial part of the myotome and clone LM82 (H,K) to its lateral part. (L-N) Examples of bisegmented unilateral clones. Clone LM53 (L) is restricted to the medial part of the myotome and clone SC81 (N) to its lateral part. Clone VG27 (M) contributes to both the medial and to the lateral parts of the myotome and is contiguous across the morphological identation of the body wall. The dashed line indicates the morphological indentation of the body wall, which marks the limit between the epaxial and hypaxial domains of the myotome. (O) Contribution of the monosegmented and bisegmented clones to the thoracic segments of the embryo. For each of thoracic segments 12-24, we calculated the numbers of monosegmented and bisegmented, unilateral clones that contain ß-gal+ cells in this segment. m, medial; l, lateral; nt, neural tube.