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Fig. 5. Regulation of minc expression. RNA in situ hybridisation using LD 47926 and anti-ß-galactosidase staining. (A) Control (twistID96/TM3lac) and (B) twistID96 embryos, showing minc expression is absent from twist mutant embryos. (C) Control (N55e11/FM7lac) and (D) N55e11 embryos to show marked reduction of minc expression in the N mutant. (E) Control (rP298; DlFX3/TM3lac) and rP298;DlFX3 to show residual minc expression as in D and enlarged population rP298-expressing (brown) founders, which do not express minc.