Fig. 5. Eyc misexpression increases R cell ER and inhibits rhodopsin delivery to the rhabdomere. In parallel confocal (A,B) and electron microscope (C,D) preparations, Hs-GAL4/UAS-eyc flies (A,C) and Hs-GAL4/+ (B,D) were given three pulses of 1 hour 37°C heat shock with 5 hours 25°C recovery starting at 70% p.d. After the last recovery, animals were dissected and processed for parallel experimental preparations. For confocal microscopy, retinal whole-mounts were stained using rhodamine-phalloidin (red) and 4C5 anti-Rh1 antibody (green). (A) Eyc overexpression results in rhodopsin accumulation in the cytoplasm and diminished delivery to the rhabdomere. (B) Rhodopsin localization is normal in heat-shocked controls; the central R7 rhabdomere does not express Rh1 and consequently only stains with phalloidin. (C) ER accumulates in photoreceptors overexpressing Eyc. (D) ER is normal in parallel heat-shocked Hs-GAL4/+ controls. Scale bars: in A, 5 µm in A,B; in D, 2 µm in C,D.