Fig. 3. Expression profile of the –4.5/+40 Krox20/lacZ transgenic line. (A-D) Transgenic embryos stained for ß-galactosidase activity at 10.5 (A), 15.5 (B) and 18.5 (C,D) dpc (hindlimb, C; thorax, D). (E) Transverse section from the distal sciatic nerve at P3 prepared by cryosectioning and subsequent staining for ß-galactosidase. (F) Teased preparation of the sciatic nerve from a P30 mouse stained for ß-galactosidase and immunolabelled with an antibody against GFAP (red-brown deposit). Preparations in E and F were counterstained with Nuclear Fast Red. The scale bars represent 25 µm.