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Fig. 1. Homozygous {alpha}8 knockout mice developed microphthalmia with mild nuclear cataracts. (A) Physical maps of the wild-type Gja8 allele, the {alpha}8 targeting vector, and the disrupted Gja8 allele. The DNA probe used for Southern blotting is indicated on the corresponding region of the wild-type and knockout Gja8 alleles. A 7.5 kb band corresponded to the knockout allele, and a 3.8 kb band for the wild-type allele. (B) The upper panel shows a blot of an embryonic stem cell clone with a disrupted Gja8 allele (+/–), in comparison with the wild-type control (+/+). This was verified by Southern blotting using a DNA probe, labeled in A. The lower panel shows the PCR results from homozygous {alpha}8 knockout (–/–), heterozygous {alpha}8 knockout (+/–) and wild-type (+/+) mice. The left arrow indicates a 320 bp band from the wild-type allele and the right arrow for a 450 bp band from the knockout allele. (C) Phenotypic comparison of the eyes and lenses from homozygous {alpha}8 knockout (–/–), heterozygous {alpha}8 knockout (+/–), and wild-type (+/+) sibs. Scale bar, 1 mm.