Fig. 5. Importance of the 19 amino acids located between the EGF and TM domains for Grk activity. (A) Structure of mbGrk19AA^myc. (B) 7% (n=334) of the eggs laid by UASp-mbGrk19AA^myc/nos-Gal4 females present a ventralized eggshell phenotype as shown by the fusion of the dorsal appendages. (C) Western blot probed with anti-Myc antibody reveals that, unlike mbGrk^myc, mbGrk19AA^myc is not cleaved when overexpressed in the oocyte (compare with Fig. 4E). Lane 2 is a protein lysate from UASp-mbGrk19AA^myc/nos-Gal4 ovaries. Only the higher band around 68 kDa that corresponds to the full-length mbGrk19AA^myc, is detected (arrow). This band is absent in wild-type ovaries (lane 1). (D) Confocal section showing a reduction of the uptake of extracellular Grk in follicle cells after overexpression of UASp-mbGrk19AA^myc in the oocyte (compare with Fig. 4C). Staining was carried out using an anti-Grk antibody.