Fig. 6. Synergy between mbGrk, Star and Rho-related proteins during oogenesis. (A) Reduction of S by expressing an antisense UASp-S in the germline causes the ventralization of the eggshell (88%, n=225). (B) Dorsal appendages are fused in S mutant follicle cell clones. (C) Misexpression of UAS-mbGrk in S/+ follicle cells results in a complete suppression of the mbGrk phenotype (compare with Fig. 1C). (D) Co-expression of UAS-S and UAS-mbGrk with CY2-Gal4 causes a strong eggshell dorsalization phenotype. (E) Ectopic expression of UAS-brho in the follicle cells using CY2-Gal4 causes a weak dorsalized eggshell phenotype. (F) Co-expression of UAS-mbGrk and UAS-brho in follicle cells induces a strong dorsalization of the eggshell. (G) Strong dorsalization of the eggshell after overexpression of mbSpi in the oocyte using pCOG-Gal4. (H) Ectopic expression of secSpi in the oocyte using the same driver causes a weaker dorsalized eggshell phenotype (33% of the eggs, n=167).