Fig. 3. Yps and Exu proteins are localized independently of one another. Ovaries in A and B are from female flies carrying a gfp-exu transgene. (A) GFP-Exu in control egg chambers illustrates the wild-type localization state of the protein. Exu accumulates in the oocyte from early oogenesis, and during mid-oogenesis becomes concentrated at the anterior and posterior poles of the oocyte. Within the nurse cells, Exu is localized to cytoplasmic particles. (B) GFP-Exu is expressed and localized normally in yps^JM2/Df(3L)BK9 ovaries. (C) Control ovaries labeled with an antibody to Yps reveal that, like Exu, Yps accumulates early in the oocyte, becomes concentrated at the anterior and posterior poles of the oocyte during mid-oogenesis, and is associated with particles in the nurse cell cytoplasm. Yps is also expressed in the follicle cells, unlike Exu. (D) In exu^SC02/exu^SC02 ovaries, Yps is expressed and localized normally.