Fig. 4. The Daf-9 phenotype is sterol dependent. (A) DAF-9 is required for normal non-dauer development. Recovery of daf-9(m540) from dauer-like arrest was assayed at 20°C on NG agar plates with cholesterol concentrations as shown. Data are presented as percent recovery on a given day after dauer-like arrest. (B) Cholesterol limitation had no effect on the recovery of the daf-7(e1372) mutant, which is Daf-c due to reduced TGF-ß signaling. (C) 7-dehydrocholesterol substituted for cholesterol to promote the recovery of daf-9(m540).