Fig. 8. Migration of PGCs towards their final target is defective in han mutants. (A-D) Dorsal views of the trunk region of embryos at day 1 of development with the PGCs stained with nos1 in blue. The PGC clusters are dispersed and located more anterior in han mutants (B,D) than in wild-type (A,C) relative to the wt1-expressing glomerulus (arrows in A,B) and relative to the endocrine pancreas stained in red with preproinsulin (C,D). (E,F) Lateral views of wild-type (E) and han mutant (F) embryos at the 25-somite stage stained with pax2.1 in blue. Note that a gap of pax2.1 expression in the pronephric mesoderm is present in wild-type embryos at the anterior end of the yolk extension (bracket in E), while expression in this region is stronger in han mutants (bracket in F).