Fig. 5. Molecular cloning of the SEUSS gene. (A) A physical map of the SEU region on Arabidopsis chromosome I. Percentage recombination for five CAPS markers is shown. n indicates the number of meiotic products examined at the given marker. BAC clones are shown as open boxes. The SEU gene, shown as a shaded box, maps 0.16 cM north of the CAPS marker F9L16Sp6. (B) Nucleotide sequence and the predicted amino acid sequence of SEU. Numbers on the right indicate the amino acid residue. The boxed area encodes a bi-partite nuclear localization signal. The underlined sequence is the putative dimerization domain with similarity to the Ldb proteins. The filled triangles indicate seu-1 and seu-2 mutations. The seu-1 allele is caused by a C to T transition, resulting in a ‘TAA’ stop codon at amino acid 501. The seu-2 allele is caused by a single base-pair deletion of the ‘G’ base indicated. The full length cDNA sequence (3555 bp) including 5' and 3' UTR has been deposited with GenBank (AF378782).