Fig. 7. ß-catenin/P-MAPK staining of stage 16 neurula, parasagittal section. (A) Original ß-catenin image; (B) ß-catenin nuclear image; (C) P-MAPK nuclear image; (D) overlay of ß-catenin and P-MAPK nuclear images. Note the overlapping staining (asterisk) in the posterior circumblastoporal collar (cc) and the complementary patterns in the brain (arrowhead and carets). The arrow indicates the midbrain-hindbrain boundary. Star, posterior endodermal ß-catenin staining; en, endoderm; ep, epidermis; fb, forebrain; md, midbrain; hb, hindbrain; np, neural plate; sm, presomitic mesoderm.