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Fig. 6. MASK is not required for MAPK activation or translocation. (A) A stacked confocal image of a wild-type third instar eye imaginal disc stained with {alpha}-diphospho-MAPK. In all panels an arrow indicates the location of the morphogenetic furrow; arrowheads mark nuclei containing activated MAPK. (B) A single confocal slice showing cytoplasmic activated MAPK in a mask10.22 clone. (C) A single confocal slice showing nuclear activated MAPK in a mask10.22 clone. (B',C') High magnification of {alpha}-diphospho-MAPK staining. (B'',C'') GFP (B''',C''') Color merge. (D) mask10.22 suppresses the ectopic R7 phenotype of rlSEM.