Fig. 4. Transdifferentiation of hair follicles in K14NLef1 transgenic animals. Hair follicles from K14NLef1 transgenic (A-C,E) and wild-type (D) mice during anagen of the first postnatal hair cycle. (A) Haematoxylin and Eosin staining; arrows indicate residual hair shaft; arrowheads indicate developing cyst. (B) Immunofluorescence staining with antibody to keratin 10 (green) and DAPI counterstain (red). MC, matrix cells; ORS, outer root sheath. Arrows and arrowheads show individual keratin 10-positive cells. (C) In situ hybridisation for Shh; arrows indicate positive signals in hair follicles and arrowheads indicate early cysts that do not express Shh. (D,E) Scanning electron micrographs of plucked hairs. Scale bars: 50 µm in A,B; 100 µm in C, 20 µm in D,E.