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Fig. 6. iro1 and iro7 act together as repressors in neural crest formation. A combination of both morpholinos (MO1+7) causes a strong reduction of fkd6 expression at an early somite stage (D) compared with either single morpholino MO1 (B), MO7 (C) or uninjected control embryos. (E) fkd6 expression is recovered (red arrowhead) by {Delta}Niro1 and {Delta}Niro7 mRNA co-injection in MO1+MO7-injected embryos. (F, G) Expression of fkd6 in hdl mutants (F) is expanded to the anterior but its expression is lost in MO1 and MO7 co-injected hdl mutants (G). Injection of iro1 (H) and iro7 (I) mRNA does not induce ectopic fkd6 expression; however, En-iro7HD mRNA induces a little ectopic fkd6 expression in the anterior neuroectoderm (J, arrowhead). By contrast, injection of VP16-iro7HD mRNA represses endogenous expression of fkd6 (K). The distribution of injected mRNA is visualized with red salmon-Gal staining. All embryos are viewed from dorsal side and anterior is towards the left.