Fig. 4. Percentages of seminiferous tubules exhibiting an abnormal expression pattern of TIF1ß or displaying vacuoles in TIF1ß^L2/L2:PrP-Cre-ER^T(0/0) (control) and TIF1ß^L2/L2:PrP-Cre-ER^T(tg/0) (experimental) mice. The percentage of seminiferous tubules cross sections showing abnormal expression of TIF1ß (i.e. partial staining of spermatocyte and/or spermatid layers along the circumference of a given tubule, instead of staining along its whole circumference) was determined by immunohistochemistry 2 weeks after tamoxifen treatment (left). The percentage of degenerating (vacuolated) versus normal tubules was determined 8 weeks after tamoxifen treatment (right). Results are mean±s.e.m. from at least three animals of each genotype and age (***P<0.0001 according to Fischer’s PLSD).