
Fig. 6. Tunnel labeling (A-D) of the testis and histological detection (E,F) of immature germ cells in the epididymis of TIF1ßL2/L2:PrP-Cre-ERT(0/0) control and TIF1ßL2/L2:PrP-Cre-ERT(tg/0) experimental males 8 weeks after tamoxifen treatment. The green fluorescent signal corresponds to nuclei containing DNA fragments. Arrowheads indicate exfoliated round spermatids. E, epithelium of the cranial portion of the epididymis; ES, elongated spermatids; P, pachytene spermatocytes; RS, round spermatids; S, Sertoli cells; SZ, spermatozoa; T, seminiferous tubules. Scale bar: 100 µm in A,B; 20 µm in C-F.