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Fig. 5. Tsh is not repressed in wg mutant discs in second instar. (A,B) Second instar wg1/wg17en40lacZ wing disc labeled to visualize Tsh (green), Vestigial (red) and wg-lacZ (blue). (B) An optical section perpendicular to the lane of the section in A. (C) Third instar disc of the same genotype labeled to visualize Wg (blue) and Tsh (red). Note the uniform expression of Tsh and the symmetric duplication of the characteristic notum stripe of Wg expression.