Fig. 6. Tsh is repressed by Wg signaling. arm^s10-expressing clones were induced in y w act>CD2>Gal4/y w HSflpI; UAS-arm^S10 /UAS-GFP larvae 24-48 hours after egg laying (A), 48-72 hours after egg laying (B,C) and 72-96 hours after egg laying (D). arm^s10-expressing clones were labeled by co-expression of GFP (red). (A) Optical section of an early third instar wing disc containing a clone induced in first instar. Tsh (green) was repressed autonomously in the clone. Hth (blue) was not. Tsh and Hth channels shown separately at right. (B) Optical section of a mid third instar wing disc containing multiple arm^s10-expressing clones. Higher magnification views of the boxed region are shown on the right. Tsh (green) was repressed in arm^s10-expressing clones (e.g. arrows). Hth (blue, or white in single channel) expression was not reduced in these clones. In some clones, Hth was upregulated. (C) Optical section of a late third instar wing disc containing multiple arm^s10-expressing clones labeled with anti-Tsh and anti-Nubbin (blue, or white in single channel). Most clones repressed Tsh but did not express Nubbin (e.g. arrow). Some clones expressed low levels of Nubbin (arrowhead). (D) Optical section of a late third instar wing disc containing multiple arm^s10-expressing clones labeled with anti-Tsh and anti-Vestigial (blue, or white in single channel). Vestigial was expressed in most clones although not in every cell of the clone (arrowheads). Some clones do not express Vestigial (arrow). Tsh was repressed in cells lacking ectopic Vestigial expression.