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Fig. 7. GAL-GCN5 at blastoderm causes loss of silencing memory in larvae. (A) BHL4G4 expression in early embryos (left) and after germ band retraction (right) with no induction of the GAL-GCN5 protein (no hs). GAL-GCN5 induction at 2-3 hours does not affect the expression of BHL4G4. (B) When both GAL-GCN5 and hs-LexA-PC (hs-LexA-PC) proteins are produced at 2-3 hours, repression induced by hs-LexA-PC is prevented by GAL-GCN5, resulting in a strong ectopic expression in late embryos. In contrast, when {alpha}1-tubulin-LexA-PC ({alpha}1T-LexA-PC) is provided maternally, GAL-GCN5 does not interfere with PcG silencing. (C) When targeted to BHL4G4PRE in the 2-3 hours embryo, GAL-GCN5 does not interfere with repression in late embryos but results in loss of repression in larval imaginal discs. When the TRX level is reduced (trx), targeting GAL-GCN5 to BHL4G4PRE leads only to a very weak and partial derepression in larvae.