Fig. 6. The ZNPC forms normally in wild-type and Math1^–/– embryos. Adjacent sections from E14.5 embryos were stained with antibody to BrdU (A,B), p27^Kip1 (C,D) or Jagged1 (E,F) to reveal the formation of the zone of non-proliferating cells within the cochlear duct of wild-type (A,C,E) and Math1^–/– (B,D,F) animals. BrdU was injected into timed-mated pregnant females 8 hours prior to sacrifice. Brackets indicate the ZNPC seen as the absence of BrdU staining in cells that have withdrawn from the cell cycle (A,B), and the site of p27^Kip1 staining (C,D). Brackets in E,F were placed after overlaying sections A-D above. The Jagged1-positive domain is largely medial (left) to the ZNPC, but appears to overlap the ZNPC slightly at its lateral edge. Arrows in E,F indicate the boundaries of the Jagged1 expression domain. Scale bar: 50 µm.