Fig. 5. Expression of AG RNA in wild type (A,B), strong LFY:VP16 (C,D), ag-1 (E,F) and strong LFY:VP16 ag-1 (G,H). Left column shows sections of shoot apices with inflorescence meristems (indicated by asterisks) surrounded by young flowers. Right column shows sections of individual flowers. Numbers indicate floral stages (Smyth et al., 1990). AG is activated precociously and ectopically in LFY:VP16. (F,G) Mutant AG RNA persists in the center of ag-1 and LFY:VP16 ag-1 flowers (arrowheads). (H) Mutant AG RNA disappears from the center of LFY:VP16 ag-1 flowers only at a late stage, after the reverted meristem has begun to produce secondary flowers (inset in H, compare with inset in F, arrows). Scale bar in H represents 50 µm for all panels, except for insets in F and H (24 µm).