Fig. 6. Expression of TFL1 RNA in wild type (A-C) and strong LFY:VP16 (D-F) plants. Left column shows sections of shoot apices with inflorescence meristems (indicated by asterisks). Middle and right columns show sections of individual flowers. Numbers indicate floral stages (Smyth et al., 1990). In wild type, TFL1 RNA is restricted to a group of subapical cells in the inflorescence meristem, and absent from flowers (arrowhead in A). In LFY:VP16 flowers, there is ectopic TFL1 expression, initially in a pattern similar to that in the shoot apical meristem (arrowheads in D,E). In the more advanced flower in (F), there is weak TFL1 expression at the tip of the gynoecium (arrow). Occasionally, as in this flower, there is also a small group of TFL1-expressing cells at the base of the central gynoecium (arrowhead), possibly indicating a group of persisting meristematic cells. Scale bar in F represents 50 µm (A,C,D,F), and 24 µm (B,E).