Fig. 7. Zpg protein is localized on the surface of germ cells in the ovary. (A) Wild-type ovariole through stage 8-9, stained with anti-Zpg (green) and anti-Fasciclin-III (red) plus anti--Spectrin (red). (B-D) Wild-type egg chambers, (B) stage 6 and (C,D) stage 7, stained with (B,C) anti-Zpg or (D) anti-Zpg sera pre-adsorbed with the C-terminal Zpg peptide immunogen. Plaques of Zpg staining (arrows) at the nurse cell-follicle cell interface; diffuse Zpg staining (arrowheads) at the nurse cell-nurse cell interface. (E,F) Wild-type germaria stained with anti-Zpg antisera. In both cases, anti-Zpg antibody stained the surface of early germ cells in the germarium. Zpg staining (arrowheads) in the dividing cysts. (E) Anti-Zpg (green) and anti--Spectrin (red). (F,I,J,L,M) Under conditions with lower levels of overall staining, Zpg protein was detected in a discrete dot (arrow) adjacent and just apical to the spectrosome in female germline stem cells. (G) Wild-type germaria stained with anti-Zpg sera pre-adsorbed with the C-terminal Zpg peptide immunogen. (H-M) High magnification views of the apical tip of two different wild-type germaria co-stained with anti-Zpg (green) (I,J,L,M) and anti-Fasciclin III plus anti--Spectrin (H,J,K,M) (red). (J,M) Merged images of H,I and K,L respectively. Dots of Zpg staining at the germline stem cell-cap cell interface (arrows).