Fig. 9. Loss of female germline cells in stem cell niche with age in zpg mutants. (A) Wild-type tip of an ovariole. (B,C) Whole ovaries from newly eclosed (B) and 3-week-old (C) zpg^z–5352/Df(3L)Zn47 females labeled with anti-Vasa (green) and anti-Fasciclin-III (red). A-C are at same magnification. Terminal filament (arrow). (D) Percent of germaria containing germ cells at the tip for 0- to 3-day-old versus 21- to 24-day-old zpg/Df females. Blue bars: zpg^z–5352/Df(3L)Zn47 (n=324 germaria from 0- to 3-day-old females, n=415 germaria from 21- to 24-day-old females). Black bars: zpg^z–5352/Df(3L)CH12 (n=388 from 0- to 3-day-old females, n=430 from 21- to 24-day-old females). Yellow bars: zpg^z–5352/Df(3L)CH20 (n=526 from 0- to 3-day-old females, n=389 from 21- to 24-day-old females).