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Fig. 1. Expression from EP(3)1076 or UAS-pygo suppresses a Wg signaling-dependant small eye phenotype. Micrographs of adult Drosophila eyes all containing P[GMR-Gal4] and the following additional transgenes: (A) P[UAS-lacZ] (two copies); (B) P[UAS-wg], P[UAS-lacZ]; (C) P[UAS-wg], EP(3)1076; (D) P[UAS-wg], P[UAS- pygo]1–1; (E) P[GMR-arm*], P[UAS-lacZ]; (F) P[GMR-arm*], EP(3)1076. Expression of wg with the GMR promoter produces an eye that is severely reduced in size (B) that is prevented by EP(3)1076 or P[UAS- pygo] (C,D). Expression of an activated form of Arm (Freeman and Bienz, 2001) causes a similar reduced eye (E) that is dramatically suppressed by EP(3)1076 (F) or P[UAS- pygo] (data not shown). Unless otherwise noted, each transgene is present in one copy/fly and cultures were reared at 25°C.