Fig. 8. jing mutations disrupt midline neuronal differentiation. Wild-type (A,C,E,G,I) and jing³ mutant embryos (B,D,F,H,J) stained with neuronal specific antibodies. Sagittal views of whole-mount stage 14 (A,B) and stage 10 embryos (I,J). (C-H) Frontal views of dissected stage 15 nerve cords with anterior up. (A) mAb 22C10 stains the VUM neuron cell bodies and axons projecting dorsally (arrow) in each nerve cord segment. (B) Absence of VUM cell bodies and axons in some jing³ segments (arrow). (C) Six wild-type Engrailed (EN)-positive neurons (detecting the VUMs and MNB). (D) Absence of EN-positive neurons in the CNS midline of some jing³ segments (arrow) and reductions in others. Neuroectodermal EN-positive neurons are not reduced from wild-type. (E,F) Wild-type P223 enhancer trap expression in the MP1, vMP2 and dMP2 neurons. (F) Downregulation of P223 expression in MP lineages of jing³ homozygotes. (G) Wild-type Odd-skipped (ODD) expression in MP1 and dMP2 neurons. (H) Reduced ODD expression in jing³ MP1 and dMP2 neurons. (I) Stage 10 embryo double-labeled with anti-ODD and TUNEL. Apoptotic MP lineages are not detectable. (J) Stage 10 jing³ homozygous mutant embryo double-labeled with anti-ODD and TUNEL. Note that ODD immunoreactivity is significantly reduced compared with wild type. TUNEL-positive MP neurons are not present in this embryo.