Fig. 5. Sensory neurons, but not autonomic neurons, are eliminated by ngn1 MO. (A,C,E,G,I) Uninjected, wild-type embryos; (B,D,F,H,J) embryos injected with 1.5 ng of ngn1 MO. (A,B) Lateral trunk views of 4 dpf embryos, processed to reveal anti-Hu immunoreactivity. Embryos have been deliberately under-developed to avoid strong staining in spinal cord underlying DRG neurons. Hu-labeled DRG neurons are absent from injected embryos, although spinal cord stain is beginning to become visible. (C,D) Lateral view just posterior to eye of 28 hpf embryos processed to reveal zn12 and anti-acetylated tubulin immunoreactivity. Trigeminal (tri), anterior lateral line (all), acoustic (ac) and posterior lateral line (pll) cranial ganglia are all highly reduced in injected embryos (28 embryos scored). (E,F) Lateral views of gut lumen of 4 dpf embryos processed to reveal anti-Hu immunoreactivity. Enteric neurons are present in both untreated and injected embryos. (G,H) Transverse sections, near axial level of pectoral fins, of 5 dpf embryos processed to reveal anti-tyrosine hydroxylase immunoreactivity. Label ventral to notochord (n) is in sympathetic neurons (arrowheads), which are present in both untreated and injected embryos. Asterisk indicates autofluorescence in a blood cell. (I,J) Lateral head view of live embryos at 5 dpf. Asterisk indicates permanently open mouth of injected embryos. Scale bars: in B, 50 µm in A,B; in D, 50 µm in C,D; in F, 50 µm in E,F; in H, 50 µm in G,H; in J, 100 µm in I,J.