Fig. 6. Expression of neurectoderm markers in EPL cell-derived neurectoderm. cDNA was synthesised from 1 µg of total RNA isolated from EB⁹, EPLEB⁹, EBM⁹ and 10.5 d.p.c. mouse embryo (used as a positive control), or EB⁹, EBM⁸, EBM⁹ and 8.5 d.p.c. mouse embryo (used as a positive control), and used as a template for PCR analysis of the genes denoted. Expression of actin was used as an example of a gene expressed in all cell types to normalise the PCR reaction. Primer sequences and product sizes can be found in the Materials and Methods.