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Fig. 2. Characterisation of eSRS21 protein using an anti-eSRS21 antibody. (A) Western blot analysis of the immature cultivated eel testis under non-reducing (lane 1) and reducing (lane 2) conditions. (B) Testicular immunoprecipitation using anti-eSRS21 (lane 1). Lane 2 shows the negative control without testicular samples. H and L indicate heavy and light chains of IgG, respectively. (C) Western blot analysis of CHO cells transfected with pSD(X)/esrs21c19 (lane 1) and its conditioned medium (lane 2) under reducing conditions. Numbers on the left represent molecular size markers (kDa).